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Selected publications

NANOPARTICLES

  • Zanzoni S, Ceccon A, Assfalg M, Singh R, Fushman D, D'Onofrio MPolyhydroxylated [60]fullerene binds specifically to functional recognition sites on a monomeric and a dimeric ubiquitin. Nanoscale 2015; 7(16):7197-7205.
  • Assfalg M, Ragona L, Pagano K, D'Onofrio M, Zanzoni S, Tomaselli S, Molinari HThe study of transient protein-nanoparticle interactions by solution NMR spectroscopy. Biochim Biophys Acta 2015.
  • Ceccon A, Lelli M, D'Onofrio M, Molinari H, Assfalg MDynamics of a globular protein adsorbed to liposomal nanoparticles. J Am Chem Soc 2014; 136(38):13158-61.

PROTEIN-MEDIATED INTRACELLULAR LIPID TRANSPORT

  • Favretto F,Santambrogio C, D'Onofrio M, Molinari H, Grandori R, Assfalg M. Bile salt recognition by human liver fatty acid binding protein. FEBS J. 2015; 7(16):7197-205.
  • Favretto F, Assfalg M, Gallo M, Cicero DO, D'Onofrio M, Molinari H. Ligand binding promiscuity of human liver fatty acid binding protein: structural and dynamic insights from an interaction study with glycocholate and oleate. Chembiochem. 2013; 14(14):1807-19.
  • Favretto F, Assfalg M, Molinari H, D'Onofrio M. Evidence from NMR interaction studies challenges the hypothesis of direct lipid transfer from L-FABP to malaria sporozoite protein UIS3. Protein Sci. 2013; 22(2):133-8.

PROTEIN-TARGETED LIPID-FUNCTIONALIZED GADOLINIUM CHELATES

  • D'Onofrio M, Gianolio E, Ceccon A, Arena F, Zanzoni S, Fushman D , Aime S, Molinari H, Assfalg M. High relaxivity supramolecular adducts between human liver fatty acid binding protein and amphiphilic Gd(III) complexes [...]. Chem Eur J . 2012; 18(32):9919-28.
  • Tomaselli S, Zanzoni S, Ragona L, Gianolio E, Aime S, Assfalg M, Molinari H. Solution structure of the supramolecular adduct between a liver cytosolic bile acid binding protein and a bile acid-based gadolinium(III)-chelate [...]. J Med Chem. 2008 Nov 13;51(21):6782-92.
  • Assfalg M, Gianolio E, Zanzoni S, Tomaselli S, Russo VL, Cabella C, Ragona L, Aime S, Molinari H. NMR structural studies of the supramolecular adducts between a liver cytosolic bile acid binding protein and gadolinium(III)-chelates bearing bile acids residues [...]. J Med Chem. 2007 Nov 1;50(22):5257-68.

STRUCTURAL STUDIES OF PILUS ASSEMBLY IN GRAMPOSITIVE BACTERIA

  • Cozzi R, Zerbini F, Assfalg M, D'Onofrio M, Biagini M, Martinelli M, Nuccitelli A, Norais N, Telford JL, Maione D, Rinaudo CD. Group B Streptococcus pilus sortase regulation: a single mutation in the lid region induces pilin protein polymerization in vitro. FASEB J. 2013; 27(8):3144-54
  • Cozzi R, Nuccitelli A, D'Onofrio M, Necchi F, Rosini R, Zerbini F, Biagini M, Norais N, Beier C, Telford JL, Grandi G, Assfalg M, Zacharias M, Maione D, Rinaudo CD. New insights into the role of the glutamic acid of the E-box motif in Group B Streptococcus pilus 2a assembly. FASEB J. 2012; 26(5):2008-18
  • Cozzi R, Malito E, Nuccitelli A, D'Onofrio M, Martinelli M, Ferlenghi I, Grandi G, Telford JL, Maione D, Rinaudo CD. Structure analysis and site-directed mutagenesis of defined key residues and motives for pilus-related sortase C1 in group B Streptococcus. FASEB J. 2011 Jun; 25(6):1874-86

METABOLOMICS

  • Ghini V, Saccenti E, Tenori L, Assfalg M, Luchinat C. Allostasis and resilience of the human individual metabolic phenotype. J Proteome Res 2015; 14(7):2951-62
  • Del Coco L, Assfalg M, D'Onofrio M, Sallustio F, Pesce F, Fanizzi FP, Schena FP. A proton nuclear magnetic resonance-based metabolomic approach in IgA nephropathy urinary profiles. Metabolomics 2013; 9:740-51
  • Napoli C, Sperandio N, Lawlor RT, Scarpa A, Molinari H, Assfalg M. Urine metabolic signature of pancreatic ductal adenocarcinoma by 1H-Nuclear Magnetic Resonance: identification, mapping, and evolution. J Proteome Res. 2012 Feb 3; 11(2):1274-8